目的：检测北京市零售药店僵蚕饮片表面所含真菌的种类，为临床安全用药提供参考依据。方法：培养僵蚕表面真菌，进行纯化，获得单一菌株。结合真菌菌落形态、显微结构特征、DNA条形码三种方法鉴 定僵蚕表面真菌。结果：从僵蚕饮片表面共获得真菌227株，共计10种。其中的8种真菌得到准确鉴定结果，2 种真菌为未知菌株。结论：初步了解北京市零售药店僵蚕表面真菌情况，为临床安全用药提供参考依据。

Objective: To detect the fungi kinds on the surface of Bombyx bassiana decoction in Beijing retail drugstore and to provide reference for safe drug use in clinic. Method: The surface fungi of Bombyx bassiana were cultivated and purified, and a single strain was obtained. Colony morphologies, microscopic characteristic and DNA barcode were combined to identify fungi on the surface of Bombyx bassiana. Result: A total of 227 strains of fungi were obtained from the surface of Bombyx bassiana, which were 10 species, among which 8 species of fungi were obtained with accurate results, 2 species were unknown strains. Conclusion: We have preliminary understanding of the surface fungi of bombyx batryticatus in Beijing retail drug stores and provide a reference for the safety of the clinical medicine.

北京市自然科学基金委面上基金项目（14G60094）：基于双重实时荧光系统监测药茶中产毒黄曲霉菌和黄曲霉素及其溯源，负责人：刘春生