目的：采用整合药理学的方法探究大黄附子汤治疗重症急性胰腺炎（severe acute pancreatitis，SAP） 的物质基础与作用机制。方法：利用中药整合药理学计算平台检索查询大黄附子汤三味中药的活性成分、药物 靶标，以及SAP的疾病靶标，建立药效成分-靶标-信号通路网络图并分析网络拓扑结构，探究大黄附子汤治疗 SAP的物质基础与作用机制。结果：平台筛选出大黄附子汤158种药效成分，主要为糖类和苷类，及659个靶 标，包括CGK、JUN、ATP1A1和KRAS等，作用的信号通路涉及炎症?内分泌?细胞?肿瘤?免疫?信号传导等 多条代谢通路。结论：大黄附子汤可能通过调节多种炎性因子和细胞因子的释放、抑制氨基端激酶磷酸化，并 调节内分泌系统和免疫活性发挥治疗SAP的作用，提示这些可能是该方治疗SAP的潜在机制和物质基础。

Objective: To explore the material basis and mechanism of treatment of severe acute pancreatitis (SAP) with Dahuang Fuzi Decoction by integrated pharmacology method. Methods: The integrated traditional Chinese medicine pharmacology computing platform was used to search and query the active ingredients and drug targets and the target of SAP of Dahuang Fuzi Decoction. The network of pharmacodynamic component-target-signal pathway was established and the network topology was analyzed to explore the material basis and mechanism of treatment of SAP with Dahuang Fuzi Decoction. Results: The platform screened 158 medicinal components of Dahuang Fuzi Decoction, which mainly consisted of carbohydrates and glycosides and 659 targets including CGK, JUN, ATP1A1, and KRAS, and the signaling pathways involved inflammation-endocrine-cell-tumor-immuno-signaling and other metabolic pathways. Conclusion: Dahuang Fuzi Decoction may treat SAP by inhibiting the production of various inflammatory factors and cytokines, inhibiting the phosphorylation of amino terminal kinase, and regulating the endocrine system and immune activity, suggesting that these may be the potential mechanism and material basis for the treatment of SAP.

国家自然科学基金委面上基金项目（81673801）：基于肠道菌群变化研究大黄附子汤对实验性重症胰腺炎防治作用的机理，负责人：路晓光