目的:建立同时测定厚朴药材中和厚朴酚，厚朴酚，辣薄荷基厚朴酚的含量测定方法，为不同产地厚朴药材品质评价提供依据。方法:采用Waters Acquity UPLC BEH-Cl8柱（2.1 mm × 50 mm, 1.7 μm），0.2%甲酸溶液(A) -甲醇(B)为流动相，梯度洗脱(0-2 min，65%-65% B；2-3 min，65%-75% B；3-5 min，75%-84% B；5-9 min，84%-90% B），柱温和流速分别为35℃和0.5 mL·min-1，波长294 nm，进样量1 μL。结果：3种木脂素成分实现完全分离，并与其他成分均能达到良好的分离；和厚朴酚，厚朴酚，辣薄荷基厚朴酚分别在20.3-406.0，15.2-304.0，5.6-112.0 ng与色谱峰峰面积呈良好线性关系；平均回收率（n=9）分别为91.75%，93.86%，95.15%；RSD 分别为1.75%，1.88%，1.91%。结论：该方法同时测定3种成分，简便快速，准确，且不同产区的厚朴药材中3种木脂素成分含量差异较大，可用于厚朴药材的质量控制。

Objective: To establish the method of simultaneous determination of contents of honokiol, magnolol and piperitylmagnolol in Cortex Magnoliae Officinalis from different regions, which could provide evidence for the qualitycontrol evaluation of Cortex Magnoliae Officinalis. Methods: The quantitative analysis was performed with a column ofWaters Acquity UPLC BEH-Cl8 (2.1 mm×50 mm, 1.7 μm) by UPLC-PDA, and eluted with a mobile phase of 0.2%formic acid solution (A) - methanol (B) in a gradient mode (0-2 min, 65%-65% B; 2-3 min, 65%-75% B; 3-5 min,75%-84% B; 5-9 min, 84%-90% B) under a flow rate of 0.5 mL·min-1. The column temperature was set at 35℃, and thedetection wavelength was 294 nm and the injection volume was 1 μL. Results: The 3 lignan components were completelyseparated and could be separated well from other components. The honokiol, magnolol and piperitylmagnolol showed agood linear relationship with chromatographic peak area in range of 20.3-406.0, 15.2-304.0, 5.6-112.0 ng, respectively.The average recoveries were 91.75%, 93.86%, 95.15% with the RSDs were 1.75%, 1.88%, 1.91% (n = 9), respectively.Conclusion: Contents of the 3 constituents from different place were significantly different and this method is simple andeffective, which can be used to quality control of Cortex Magnoliae Officinalis.

国家中医药管理局国家中药标准化项目(ZYBZH-Y-ZY-45)：白术等14种中药饮片标准化建设，负责人：兰青山