目的：观察中药复方制剂益气固表丸对COPD模型大鼠JAK/STAT通路及炎性反应表达影响，探讨益气固表丸治疗COPD的分子机制。方法：将60只鼠龄为8周的SPF级雄性Wistar大鼠随机分为3组，分别为：空白组、模型组和益气固表丸组，每组20只，其中模型组及益气固表丸组大鼠采用烟熏+脂多糖（LPS）气管内滴注方法建立COPD模型，空白组和模型组大鼠给予生理盐水灌胃，益气固表丸组大鼠给予益气固表丸相应剂量的水溶液灌胃，2次/天，连续12周。采用HE染色法检测大鼠肺组织形态学改变，ELISA法测定外周血中IL-17a、IL-23及INF-γ及RORγt表达的变化，RT-PCR方法测定肺组织JAK1、JAK3、STAT1、STAT3 mRNA表达的变化。结果：与对照组比较，益气固表丸组大鼠HE染色的炎症细胞浸润情况减轻，支气管管腔直径变宽，差异有统计学意义（P < 0.01）；ELISA及RT-PCR结果均显示：与空白组相比较，模型组大鼠IL-17a、IL-23、RORγt水平升高，JAK1、JAK3、STAT1、STAT3 mRNA水平升高，与模型组比较，益气固表丸组以上指标mRNA水平下降，差异有统计学意义（P < 0.01）。相关性分析提示JAK1、JAK3、STAT1、STAT3与IL-17a、IL-23及RORγt相关，具有统计学意义（P < 0.05）。结论：益气固表丸能够改善COPD模型大鼠肺内炎症反应，有效机制与抑制JAK/STAT通路活性有关。

The research observed the effect of Yiqigubiao Pill regulating inflammatory reaction mRNA expression in JAK/STAT pathway in COPD rats model and explored protective effect of Yiqigubiao pills in the treatment or auxiliarypossible mechanism in COPD. 60 SFP Wistar rats were divided randomly into control group (CN), COPD model group (M)and Yiqigubiao Pill group (Yqgb). The rats in group M and group YQGB were establish by using the method of LPS and smoking as COPD, rats in group M and group CN were given normal saline intragastric administration, and rats in the YQGB group were given water solution intragastric administration at the corresponding dose, twice a day. After using drugs for 12 weeks, we used ELISA method to detect changes inIL- 17a, IL- 23, IFN- γ and RORγt expression inperipheral blood, RT-PCR method to detect changes in the expression of JAK1, JAK3, STAT1 and STAT3 mRNA in lungtissues. Compared with the group CN, the infiltration of inflammatory cells stained by HE in the experimental group was reduced, and the diameter of the bronchial lumen was broadened (P < 0.01). Both of ELISA and RT-PCR results showed that, compared with group CN, the levels of IL-17a, IL-23 and RORγt , the levels of JAK1, JAK3, STAT1, and STAT3mRNA were increased in group M. Compared with group M, the mRNA levels of above indicators in group Yqgb were decreased, and the difference was statistically significant (P < 0.01). Correlation analysis revealed that JAK1, JAK3,STAT1 and STAT3 were associated with IL-17a, IL-23, and RORγt had statistical significance (P < 0.05). Therefore, Yiqgubiao Pill can inhibit the inflammatory response in COPD rats model, and its mechanism may be associated with inhibition of JAK/STAT pathway.

新疆维吾尔自治区科技厅重点实验室开放课题（2016D03024）：益气固表丸对慢性阻塞性肺疾病免疫调节相关转导通路的调节作用，负责人：李风森