目的 建立炮附片HPLC特征图谱，分析炮附片炮制前后物质基础差异。方法 AgilentExtend-C18(4.6 mm×250 mm，5 μm)；乙腈（A）-40 mM乙酸铵缓冲液(氨水调PH为10.5)（B），梯度洗脱，检测波长为240 nm，柱温为30℃，进样量为20 μL。结果 建立了炮附片HPLC特征图谱。生附子经炮制成为黑顺片后，11个峰的峰面积显著减小，3种单酯型生物碱含量降低，3种双酯型生物碱基本检测不到。黑顺片经砂炒制成炮附片后，3种单酯型生物碱含量进一步降低，且无新成分峰出现。结论 生附子经炮制成为炮附片后，可使生附子的成分发生量变和质变。炮附片与黑顺片特征图谱基本一致，含量略有差别。

Objective to establish a HPLC characteristic chromatogram of Radix Aconiti Lateralis Praeparata and analyze the differences in material basis before and after processing.Method Agilent Extend-C18 (4.6 mm×250 mm, 5 μm), acetonitrile (A) -40 mM ammonium acetate buffer (pH adjusted to 10.5 by aquaammonia) (B), gradient elution, detection wavelength of 240 nm, column temperature of 30℃, and injection volume of 20 μL.Results The HPLC characteristic chromatogram of Radix Aconiti Lateralis Praeparata was established. After the Aconitum Carmichaeli Debxbeing made into Heishunpian, the area of the 11 peaks was significantly decreased. The content of 3 monoester alkaloids wasdecreased, and 3 diester alkaloids were basically detectable. After Heishunpian being made into Radix Aconiti Lateralis Praeparata, the content of the 3 monoester alkaloids was further decreasedwithout new composition peaks.Conclusion After the Aconitum Carmichaeli Debxbeing made into Radix Aconiti Lateralis Praeparata, the composition of Aconitum Carmichaeli Debx can be changed quantitatively and qualitatively. The characteristicchromatograms of the Heishunpian and Radix Aconiti Lateralis Praeparata are basically the same, withslightly different content.