目的：研究桂枝茯苓胶囊（GZFLC）对实验性子宫内膜异位症痛经作用机制。方法：将60只SD雌性大鼠随机分为假手术组、模型组、散结镇痛胶囊组、GZFLC高、中、低剂量组（0.54、0.27、0.14 g?kg-1）组，每组10只。每天灌胃给药，连续28 d。观察各组扭体反应，测量移植物体积并进行病理检测，采用免疫组化法检测异位灶NGF、TRPV1 蛋白的表达。结果：与模型组比较，散结镇痛胶囊组和GZFLC高、中、低剂量组大鼠扭体次数显著减少（P ＜ 0.01），其抑制率分别为51.1%、63.2%、60.4%、47.1%。与模型组比较，散结镇痛胶囊组和GZFLC高、中、低剂量组大鼠移植物体积显著减少（P ＜ 0.01），其抑制率分别为61.9%、66.0%、59.1%、56.6%。与模型组比较，GZFLC高、中剂量组可显著降低子宫内膜异位症痛经大鼠异位组织的病理学评分及NGF、TRPV1 蛋白的表达。结论：GZFLC对实验性子宫内膜异位症痛经具有一定治疗作用，其机制与抑制NGF、TRPV1蛋白表达相关。

Objective: To study the effect and mechanism of Guizhi Fuling Capsule（GZFLC）on dysmenorrhea induced by experimental endometriosis in rats. Methods: Sixty SD female rats were randomly divided into sham operation group,model group, Sanjie Zhengtong Capsule group, GZFLC in high -, mid-, and low -dose (0.54、0.27、0.14 g ? kg-1) group,with 10 animals in each group. Rats were given drug by intragastric perfusion for 28 d. The writhing reaction wasobserved in all groups. The graft volume was measured and pathological examination was performed. Expression levels ofNGF and TRPV1 proteins in ectopic foci were detected by immunohistochemical method. Results: Compared with themodel group, the number of writhing was significantly decreased in the Sanjie Zhengtong capsule group and the GZFLCin high -, mid-, and low -dose groups (P < 0.01), and the inhibition rates were 51.1%, 63.2%, 60.4% and 47.1%respectively. Compared with the model group, the graft volume in the Sanjie Zhengtong capsule group and the GZFLC inhigh -, mid-, and low -dose groups were significantly reduced (P < 0.01), and the inhibition rates were 61.9%, 66%,59.1% and 56.6% , respectively. Compared with the model group, GZFLC high and medium dose groups couldsignificantly reduce the pathological score of ectopic tissue and the expression of NGF and TRPV1 protein inendometriosis dysmenorrhea rats. Conclusion: GZFLC has certain therapeutic effect on dysmenorrhea induced byendometriosis, and its mechanism may be related to the inhibition of NGF and TRPV1 protein expression.

国家科技部重大科技专项资助项目（2013ZX09402203）：现代中药创新集群与数字制药技术平台，负责人：王振中；江苏食品药品职业技术学院院级项目（Jsfp2018001）：基于PKC-MAPK/ERK和PLC/IP3通路的桂枝茯苓胶囊及其成分桂皮醛防治原发性痛经的作用机制研究，负责人：孙兰。