目的 探究中药复方开心散调控中枢神经炎症系统抗抑郁的作用机制。方法 制备开心散不同配伍比例提取物，采用小鼠慢性应激压力（CUMS）模型，通过糖水偏嗜实验、悬尾实验和强迫游泳实验评价开心散不同配伍比例的抗抑郁功效。采用ELISA法测定受试动物海马组织促炎物质如脂多糖（LPS）、IL-1β、IL-6、TNF-α的含量。采用免疫组织化学染色法观察受试动物海马区小胶质细胞形态。进而采用LPS促进小鼠小胶质永生化细胞（BV2）炎性因子表达细胞模型，采用ELISA法评价开心散抑制炎症因子表达的作用，采用Western blotting法考察NF-κB信号通路的调控情况。结果 开心散不同配伍比例能显著上调模型小鼠糖水偏嗜率，缩短小鼠悬尾和强迫游泳不动时间，以D-652效用最强。该配伍比例通过抑制NF-κB的入核和抑制NF-κB磷酸化等环节，下调BV2细胞中炎症因子IL-1β、IL-6、TNF-α的表达水平。结论 对中枢神经炎症的调控是中药复方开心散抗抑郁的重要作用环节。

Objective To explore the anti-depression mechanism of Kai-Xin-San via regulation of inflammation cytokine levels in hippocampus of depression-like mice.Methods The extracts of Kai-Xin-San (KXS) were prepared with different compatible ratios were prepared and the anti-depression effects of Kai-Xin-San were evaluated by behavioral tests on chronic expected mild stress (CUMS) induced depression-like mice. Amounts of LPS, IL-1β, IL-6 and TNF-α in hippocampus were determined by ELISA tests. Morphology of microglia was observed by immunohistochemistry methods in hippocampus. BV2 mouse microglia cell line was used to evaluate the effect of Kai-Xin-San on decreasing the expression of inflammatory cytokines. In addition, effects of KXS on regulating NF-κB signaling pathway were explored by Western blotting.Result Kai-Xin-San significantly ameliorated the depression-like behaviors in presence of increased sucrose preference rate and decreased immobile time of tail suspension and forced swimming. D-652 ratio showed comparative better effect in behavior tests and decreasing the expressions of inflammatory cytokines such as IL-1β, IL-6 and TNF-α in hippocampus. On BV2 cells, KXS also decreasing expressions of inflammatory cytokines by inhibiting NF-κB entering into nuclear and suppressing the phosphorylation of NF-κB.Conclusions Regulation of inflammation cytokines in hippocampus accounted for KXS exerting anti-depression effect on CUMS induced depression-like mice.