目的 采用血浆差异代谢组学的方法筛选和评价急性脑出血瘀热病机的特征代谢物，并确立生物标志物。方法 根据《中医脑出血瘀热评分量表》，采用匹配的方法收集临床瘀热型与非瘀热型急性ICH患者各40例，健康对照40例，应用液相色谱-质谱联用技术(LC-MS)检测瘀热组(YP)、非瘀热组(FP)及健康组(JP)之间血浆差异代谢物。采用主成分分析(PCA)、偏最小二乘法判别分析(PLS-DA)和正交偏最小二乘法判别分析(OPLS-DA)对代谢组学数据进行多维统计分析，筛选急性脑出血瘀热病机单元的特征代谢物。结果 YP/JP检测出27个差异代谢物；FP/JP检测出23个差异代谢物；FP/YP检测出14种差异代谢物，主要涉及类固醇激素合成、组氨酸代谢、半乳糖代谢等9个通路。结论 可的松21-乙酸酯、酸甲酯和甘油三酯可作为一组急性脑出血瘀热病机的潜在生物标志物。

Objective To screen and evaluate biomarkers of the pathogenesis of stasis-heat in Acute Intracerebral Hemorrhage based on the plasma differential metabonomics.Methods According to the rating scale of Stasis-heat in Intracerebral Hemorrhage in Traditional Chinese Medicine, 40 patients with clinical stasis-heat in acute ICH, 40 patients with clinical nonstasis-heat in acute ICH, and 40 healthy controls were collected by matching method. Plasma differential metabolites were detected by liquid chromatography-mass spectrometry (LC-MS) among the stasis-heat group, the nonstasis-heat group and the healthy group. Principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and orthogonal partial least square discriminant analysis (OPLS-DA) were used to analyze the metabonomics data multidimensional statistical analysis to screen the potential biomarkers of the pathogenesis of stasis-heat in Acute Intracerebral Hemorrhage.Results Twenty-seven differential metabolites were detected in the stasis-heat group/healthy group; twenty-three differential metabolites were detected in the nonstasis-heat group/healthy group; fourteen differential metabolites were detected in the nonstasis-heat group/stasis-heat group, involving pathways like steroid hormone synthesis, histidine metabolism and others.Conclusion Cortisone 21-Acetate, methyl reserpate and triglyceride can be considered as a set of potential biomarkers for the pathogenesis of stasis-heat in Acute Intracerebral Hemorrhage.

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