目的 对比龟鹿二仙胶配方颗粒膏与传统饮片膏中的13种氨基酸类成分以及二者对大鼠血清免疫球蛋白的影响。方法 采用柱前衍生-UPLC法分析19批龟鹿二仙胶配方颗粒膏与传统饮片膏样品，建立二者的指纹图谱，并采用标准品对照，对其中氨基酸成分进行定性分析，并取其中含量最高的几种成分进行定量分析。建立大鼠阳虚型模型，分别采用龟鹿二仙胶配方颗粒膏与传统饮片膏进行干预，检测各组大鼠血清中IgA、IgG、IgM免疫球蛋白的含量。结果 成功建立龟鹿二仙颗粒膏与饮片膏的指纹图谱，发现19批膏方的相似度接近（P > 0.99），采用标准品确定其中13种氨基酸成分，其中各批次中成分含量较高的4种氨基酸，L-羟脯氨酸、甘氨酸、丙氨酸、L-脯氨酸含量无明显差别。且二者对阳虚型大鼠血清免疫球蛋白的影响也基本相似（P > 0.05）。结论 龟鹿二仙胶配方颗粒膏较与传统饮片膏比较，氨基酸类成分的定性与主要氨基酸类成分的定量分析均无明显区别，其基本的药理作用也较为接近。但配方颗粒成膏技术较传统成膏技术更加简便与高效，具有巨大的市场潜力与发展前景，值得深入研究

Objective To compare the amino acid components in Guilu Erxian Jiaopaste and granules and their effects on serum levels of immunoglobulin in rats.Methods Pre-column derivatization-UPLC was used to analyze 19 batches of samples, the fingerprints of Guilu Erxian Jiao paste and granules were established, and analyze the amino acid composition and take quantitative analysis of several components with the highest content. A model of rats with yang deficiency was established, and Guilu Erxian Jiao paste and granules were used as interventions to detect the content of IgA, IgG and IgM immunoglobulin in the serum of rats in each group.Results The fingerprints of Guilu Erxian Jiao paste and granules were successfully established, and 19 batches had similarities (P > 0.99). The standard products were used to determine 13 kinds of amino acid components, among which the 4 amino acids with higher content were L-hydroxyproline, glycine, alanine and L-proline. There was no significant difference in the content of these amino acids. And the two have similar effects on the serum levels of immunoglobulin in rats (P > 0.05).Conclusion Compared with the traditional paste, the granules showed no obvious difference in the qualitative analysis of amino acid components and the quantitative analysis of the main amino acid components and had similar pharmacological effects. Making plasters with Guilu Erxian Jiao granules is a better choice than using the traditional paste due to its simplicity and high efficiency, which makes it worth studying.