目的 为了探索牛膝-王不留行治疗糖尿病勃起功能障碍大鼠的靶点、机制。方法 通过TCMSP数据库收集牛膝-王不留行活性成分及潜在靶点；通过GeneCards数据库和DisGeNET数据库收集糖尿病勃起功能障碍相关的疾病靶点，并与药物活性成分靶点取交集得到共同靶点，进行PPI分析；使用Cytoscape构建可视化“药物-靶点-疾病”网络并进行分析，通过R语言对共同靶点进行GO富集与KEGG富集分析。通过腹腔注射链脲佐菌素（STZ）溶液（55 mg·kg^-1）构建糖尿病大鼠模型，予以阿扑吗啡（APO）皮下注射（100 ug·kg^-1）筛选糖尿病勃起功能障碍（DMED）模型。造模成功后予以牛膝-王不留灌胃干预。通过APO诱导来评估大鼠勃起功能，运用HE染色观察大鼠阴茎海绵体组织结构变化，采用PCR和Western blotting方法检测组织相关蛋白及mRNA的表达，以验证牛膝-王不留行对DMED治疗作用及机制。结果 网络药理学研究结果表明ICAM-1和NOS是牛膝-王不留行在治疗DMED中的关键靶点。实验结果表明牛膝-王不留行可以明显的改善DMED大鼠的勃起功能，同时可以明显改善阴茎海绵体组织结构结构。与模型组相比，药对可以抑制大鼠阴茎组织中ICAM-1蛋白及mRNA的表达，并促进NOS蛋白及mRNA的表达。结论 牛膝-王不留行通过抑制ICAM-1和促进NOS蛋白及mRNA的表达，进而改善阴茎海绵体组织结构，从而调节DMED的大鼠的勃起功能。

Objective To explore the target and mechanism of Achyranthis Bidentatae Radix and Vaccariae Semen intervention in rats with diabetic erectile dysfunction.Methods The active ingredients and potential targets of Achyranthis Bidentatae Radix and Vaccariae Semen were collected through the TCMSP database; the disease targets related to diabetic erectile dysfunction were collected through the GeneCards database and the DisGeNET database, and the common targets were obtained by intersection with the active ingredient targets of the drug. PPI analysis was performed; Cytoscape was used to construct and analyze the visualized “drug-target-disease” network, and perform GO enrichment and KEGG enrichment analysis on common targets through R language. A diabetic rat model was constructed by intraperitoneal injection of streptozotocin (STZ) solution (55 mg·kg^-1), and apomorphine (APO) was injected subcutaneously in the neck (100 ug·kg^-1) to screen diabetic erectile dysfunction (DMED) models. After successful model building, Achyranthis Bidentatae Radix and Vaccariae Semen was given intragastric intervention. The erectile function of rats was evaluated by induction of APO. The structural changes of the rat penile corpus cavernosum was observed by HE staining, and the expression of tissue-related proteins and mRNA was detected by PCR and Western blotting to verify the treatment of Achyranthis Bidentatae Radix and Vaccariae Semen function and mechanism.Results The results of the network pharmacology study showed that ICAM-1 and NOS were the key targets of Achyranthis Bidentatae Radix and Vaccariae Semen in the treatment of DMED. Experimental studies showed that Achyranthis Bidentatae Radix and Vaccariae Semen can significantly improve the erectile function of DMED rats, and at the same time can significantly improve the structure of the corpus cavernosum tissue. Compared with the model group, the drug pair can inhibit the expression of ICAM-1 protein and mRNA in rat penile tissue, and promote the expression of NOS protein and mRNA.Conclusion Achyranthis Bidentatae Radix and Vaccariae Semen can improve the structure of the penile cavernous body by inhibiting ICAM-1 and promoting the expression of NOS protein and mRNA, thereby improving the erectile function of DMED rats.