目的 探究寻常型银屑病血热证和血燥证的肠道菌群差异。方法 采集寻常型银屑病血热证患者、血燥证患者以及健康人的新鲜粪便标本各15例，提取DNA、进行宏基因组学测序，分析各组肠道菌群的α多样性、β多样性以及菌群差异。结果 血热组、血燥组菌群的α多样性和β多样性较健康对照组无显著差异；不同证型银屑病患者的肠道菌群有其独特组成，其中门水平的Firmicutes，属水平的Prevotella、Butyricimonas等在血热组和血燥组中均减少，种水平的Clostridium bartlettii、Citrobacter freundi和Dialister菌属等在血热组中增多，Megamonas funiformis菌种、Cellulophaga菌属等在血燥组中增多。结论 寻常型银屑病患者的肠道菌群较健康人群发生了紊乱，血燥证较血热证菌群紊乱更严重；采用中药调整肠道菌群可作为治疗银屑病血热证和血燥证的潜在方案。

Objective To explore the differences of intestinal flora between blood-heat syndrome (Xue Re, XR) and blood-dryness syndrome (Xue Zao, XZ) of psoriasis vulgaris.Methods Fresh fecal samples were collected from 15 patients with blood-heat syndrome and 15 patients with blood-dryness syndrome of psoriasis vulgaris and 15 healthy persons. Then DNA was extracted from the faecal microbiota, and metagenomics sequencing and bioinformatic analyses were performed, including α-diversity and β-diversity and the differences of bacterial flora.Results The gut microbiota of group of XR and XZ showed no significant difference in diversity compared with healthy individuals. A distinct gut microbial composition in patients with psoriasis was observed, with an increased abundance of the phyla Firmicutes and genera Prevotella, Butyricimonas and Odoribacter. The abundance of the species Clostridium bartlettii, Citrobacter freundi and genera Dialister were decreased in group of XR, and the abundance of the species Megamonas funiformis and genera Cellulophaga were decreased in group of XZ.Conclusion Gut microbiota dysbiosis was in patients with psoriasis vulgaris, while patients with XZ showed worse dysbiosis than patients with XR. Using traditional Chinese medicine to adjust intestinal flora could be used as potential treatment methods for psoriasis.

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