目的 研究酸枣仁汤对老年慢性睡眠剥夺模型大鼠核受体PPARγ及其共激活因子PGC-1α表达的影响。方法 将50只雄性Wistar大鼠随机分为环境对照组、模型组、阳性对照组、酸枣仁汤低、高剂量组，除环境对照组外，其余各组均皮下注射D-半乳糖，于末次给药后进行多平台水环境睡眠剥夺，造模结束后各组开始灌胃给药，连续给药7天。采用自主活动仪检测大鼠光照环境活动时间、黑暗环境活动时间、总活动时间，采用比色法检测大鼠下丘脑ATP含量的变化，采用Real Time-PCR、Western-blot和免疫组化分别检测大鼠下丘脑核受体PPARγ及其共激活因子PGC-1α mRNA和蛋白表达水平。结果 与环境对照组比较，模型组大鼠光照环境活动时间、黑暗环境活动时间、总活动时间均显著增加（P < 0.01）；与模型组比较，阳性对照组、酸枣仁汤高剂量组光照环境活动时间、黑暗环境活动时间、总活动时间均显著减少（P < 0.05，P < 0.01）；与环境对照组比较，模型组下丘脑ATP含量显著降低（P < 0.01）；与模型组比较，阳性对照组、酸枣仁汤高剂量组下丘脑ATP含量显著升高（P < 0.05）；与环境对照组比较，模型组核受体PPARγ及其共激活因子PGC-1α mRNA和蛋白表达水平显著降低（P < 0.01，P < 0.05）；与模型组比较，阳性对照组、酸枣仁汤高剂量组核受体PPARγ及其共激活因子PGC-1α mRNA和蛋白表达水平显著升高（P < 0.01，P < 0.05）。结论 酸枣仁汤有可能基于PPARγ及其共激活因子PGC-1α同时调控昼夜节律与能量代谢。

Objective To study the effect of Suanzaoren Decoction on the expression of PPAR γ and PGC-1 α in aged rats with chronic sleep deprivation.Methods 50 male Wistar rats were randomly divided into environmental control group, model group, positive control group, low-dose and high-dose Suanzaoren Decoction groups. Except the environmental control group, the other groups were subcutaneously injected with D-galactose. After the last administration of D-galactose, they were subjected to multi-platform water environment sleep deprivation. After the end of modeling, each group began to give drugs by gavage for 7 consecutive days. The activity time in light environment, dark environment and total activity time were detected by autonomic activity analyzer. The content of ATP in hypothalamus was detected by colorimetry. The mRNA and protein expression levels of PPAR γ and its coactivator PGC-1 α in hypothalamus were detected by real time PCR, Western blot and immunohistochemistry.Results Compared with the environment control group, the light environment activity time, dark environment activity time and total activity time of the model group were significantly increased (P < 0.01); compared with the model group, the light environment activity time, dark environment activity time and total activity time of the positive control group and the high dose Suanzaoren Decoction group were significantly decreased (P < 0.05, P < 0.01); compared with the environment control group, the light environment activity time, dark environment activity time and total activity time of the model group were significantly increased (P < 0.05, P < 0.01). The content of ATP in hypothalamus of model group was significantly lower than that of model group (P < 0.01); the content of ATP in hypothalamus of positive control group and high-dose Suanzaoren Decoction group was significantly higher than that of model group (P < 0.05); the content of PPAR γ and PGC-1 α in model group was significantly higher than that of environmental control group. Compared with the model group, the mRNA and protein expression levels of PPAR γ and PGC-1 α in the positive control group and high-dose Suanzaoren Decoction group were significantly increased (P < 0.01, P < 0.05).Conclusion Suanzaoren Decoction may regulate circadian rhythm and energy metabolism simultaneously based on PPAR γ and its coactivator PGC-1 α.

国家自然科学基金委员会面上项目（81473560）：酸枣仁汤对基于5-HTR调控老年失眠SCN及VLPO系统的作用及机制研究，负责人：游秋云