目的 分析判断MS-DAIL与MS-FINDER联合运用以鉴定中药黄酮类化合物的可行性。方法 采用UPLC-MS/MS技术检测含有20种黄酮类化合物的混合对照品溶液，利用MS-DAIL联合MS-FINDER对特征峰进行提取、数据库比对与物质鉴定。结果 MS-DAIL与MS-FINDER联合运用可以快速获取黄酮类化合物MS/MS特征谱，与数据库中图谱进行匹配，根据得分共准确鉴定出黄酮类化合物15种，鉴定错误5种，准确率为75%，其中1种无谱图信息，有4种存在MS/MS谱相似的异构体。结论 MS-DAIL与MS-FINDER的联合使用以鉴别中药黄酮类成分高效简便，是初步鉴定黄酮类化合物的可选方式。

Objective To analyze the feasibility of MS-DAIL combined with MS-FINDER for the identification of flavonoids in Chinese herb.Methods UPLC-MS/MS was used for detecting the mixed reference solution containing 20 kinds of flavonoids. The characteristic peaks, comparison with database and substance identification were extracted by MS-DAIL combined with MS-FINDER.Results MS-DAIL combined with MS-FINDER could quickly extract and analyze the MS/MS spectrum of flavonoids. According to the score and database map, 15 flavonoids could be accurately identified, and 5 were identified incorrectly, with an accuracy rate of 75%. One of them had no spectral information, and four had isomers with similar MS/MS spectra.Conclusion The combined use of MS-DAIL and MS-FINDER to identify the flavonoids in Chinese herb is efficient and simple, and it is an optional method for preliminary identification of flavonoids.

国家自然科学基金委员会面上项目（81873340）：I型干扰素通路介导的清肺口服液类黄酮组分抗RSV的机制研究，负责人：袁斌。