目的 探究西红花苷对与成纤维细胞共培养的结直肠癌细胞生物学行为的影响，并初步探究作用机制。方法 将人结直肠癌细胞HCT116和人表皮成纤维细胞ESF-1共培养，再用低、中、高（50、100、200 μmol·L^-1）剂量西红花苷处理共培养后的HCT116细胞，实验分组包括对照组、ESF-1/HCT116组、ESF-1/HCT116 + Cro-L组、ESF-1/HCT116 + Cro-M组、ESF-1/HCT116 + Cro-H组；MTT法检测细胞增殖，PI染液法检测细胞周期分布情况，Transwell实验检测细胞侵袭与迁移能力，化学比色法检测超氧化物歧化酶（SOD）活性，硫代巴比妥酸比色法检测丙二醛（MDA）含量；荧光探针DCFH-DA测定活性氧（ROS）水平，Western blot检测转化生长因子-β（TGF-β1）、上皮性钙粘附分子（E-cadherin）、神经性钙黏附蛋白（N-cadherin）与波形蛋白（Vimentin）蛋白表达水平。结果 与共培养体系下的HCT116细胞比较，共培养后再经各浓度西红花苷处理后的HCT116细胞存活率下降，G1期细胞比例减少，G2期细胞比例增加，细胞的侵袭数目和迁移数目均减少，TGF-β1、N-cadherin与Vimentin蛋白表达水平均下调，E-cadherin蛋白表达水平上调，此外，SOD活性下降而MDA含量升高，ROS水平也明显提高，差异均具有统计学意义（P < 0.05）。结论 西红花苷可抑制与成纤维细胞共培养的结直肠癌细胞的增殖、侵袭与迁移能力，其机制可能与诱导细胞发生氧化应激损伤有关。

Objective To investigate the effect of crocin on the biological behavior of colorectal cancer cells co-cultured with fibroblasts, and to preliminarily explore the its mechanism.Methods Human colorectal cancer cells HCT116 and human epidermal fibroblasts ESF-1 were co-cultured, and then the co-cultured HCT116 cells were treated with low, medium and high (50, 100 and 200 μmol·L^-1) doses of saffron glucoside, and the experimental groups included control group, ESF-1/HCT116 group, ESF-1/HCT116 + Cro-L group, ESF-1 /HCT116 + Cro-M group, ESF-1/HCT116 + Cro-H group; MTT assay for cell proliferation, PI staining solution for cell cycle distribution, Transwell assay for cell invasion and migration ability, chemical colorimetric assay for superoxide dismutase (SOD) activity, and thiobarbituric acid colorimetric assay for malondialdehyde (MDA) content. Fluorescent probe DCFH-DA was used to determine the level of reactive oxygen species (ROS), and Western blot was used to detect the expression levels of transforming growth factor-β (TGF-β1), epithelial calcium adhesion molecule (E-cadherin), neural calcium adhesion protein (N-cadherin) and vimentin protein.Results Compared with HCT116 cells in co-culture system, after treatment with various concentrations of crocin, the survival rate of HCT116 cells decreased, the proportion of cells in the G1 phase decreased, the proportion of cells in the G2 phase increased, and the number of invasion and migration of cells decreased, the protein expression levels of TGF-β1, N-cadherin and Vimentin were down-regulated, and the protein expression level of E-cadherin was up-regulated. In addition, SOD activity decreased while MDA content increased, and ROS levels also increased significantly. The differences were statistically significant (P < 0.05).Conclusion Crocetin can inhibit the proliferation, invasion and migration of colorectal cancer cells co-cultured with fibroblasts, and its mechanism may be related to the induction of oxidative stress damage in cells.

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