目的 通过UHPLC-Q-TOF-MS代谢组学探讨艾灸关元穴对老年大鼠肾代谢物的影响，进而为艾灸关元穴的作用机制提供参考。方法 将8月龄SD雄性大鼠设为成年对照组（8只），21月龄SD雄性大鼠随机分为老年对照组（8只）、老年金匮肾气丸组（7只）、老年艾灸组（8只）。老年金匮肾气丸组每日按体重给药，老年艾灸组每日艾灸关元穴15 min，均每周5天。实验持续13周后检测大鼠肾组织线粒体呼吸耗氧速率、琥珀酸脱氢酶（SDH）活性以及血清肾功能指标，观察肾脏病理变化，结合UHPLC-Q-TOF-MS技术对大鼠的肾组织进行代谢轮廓分析，筛选代谢差异物并进行鉴定。结果 与老年对照组比较，老年艾灸组大鼠肾线粒体的呼吸耗氧速率和SDH酶的活力显著提高（P<0.01）。代谢组学结果显示，肾组织中筛选出13个共同差异化合物，分别是丁酸十二烷基酯、亚油酰胺、5-甲基四氢叶酸、PC（16∶0/22∶5（7Z，10Z，13Z，16Z，19Z））、6，8-二羟基嘌呤、1，2，3-丙烷三羧酸、3-（4-甲氧基苯基）-2-氧代丙酸、吲哚-3-乙酰甘氨酸、亚麻油酸、9，10-环氧十八烷酸、二十二碳五烯酸（22n-6）、牛磺胆酸、LysoPS （18∶0/0∶0）。结论 艾灸关元穴可通过调控老年大鼠的牛磺酸和亚牛磺酸代谢、α-亚麻酸代谢、亚油酸代谢、甘油磷脂代谢来调节肾的能量代谢。

Objective To explore the effect of moxibustion at Guanyuan (CV4) on renal metabolites in aged rats by UHPLC-Q-TOF-MS metabonomics, so as to provide reference for the action mechanism of moxibustion at Guanyuan point.Methods 8-month-old SD male rats were set as adult control group (n=8), and 21-month-old SD male rats were randomly divided into aged control group (n=8), aged Jinkui Shenqi pill group (n=7) and aged moxibustion group (n=8). The aged Jinkui Shenqi pill group was given medicine according to body weight daily, the aged moxibustion group was given moxibustion at Guanyuan point for 15 min daily, and the other groups were treated with the same treatment except without moxibustion. After the experiment lasted for 13 weeks, the respiratory oxygen consumption rate of rat kidney mitochondria, the activity of succinate dehydrogenase (SDH) and the index of serum renal function were detected, and the pathological changes of kidney were observed. The metabolic profile of rat kidney tissue was analyzed by UHPLC-Q-TOF-MS technique, and the metabolic differences were screened and identified.Results Moxibustion at Guanyuan can increase the respiratory oxygen consumption rate (P<0.01) and the activity of SDH enzyme in aged rats (P<0.01) of renal mitochondria. The metabonomic results showed that 13 potential biomarkers were screened from the renal tissue, which were Dodecyl butyrate, Linoleamide, 5-Methyltetrahydrofolic acid, PC(16∶0/22∶5(7Z,10Z,13Z,16Z,19Z)), 6,8-Dihydroxypurine, 1,2,3-Propanetricarboxylic acid, 3-(4-methoxyphenyl)-2-oxopropanoic acid, Indole-3-acetylglycine, Linolenelaidic acid, 9,10-Epoxyoctadecanoic acid, Docosapentaenoic acid (22n-6), Taurocholic acid, LysoPS(18∶0/0∶0). The metabolic pathways involved are Linoleic acid metabolism、Taurine and hypotaurine metabolism、One carbon pool by folate、α-Linolenic acid metabolism、Arachidonic acid metabolism、Glycerophospholipid metabolism、Primary bile acid biosynthesis.Conclusion Moxibustion at Guanyuan point can regulate the renal energy metabolism by regulating the metabolism of taurine and hypotaurine metabolism, α-linolenic acid metabolism, linoleic acid metabolism and glycerophospholipid metabolism in the aged rats.

国家中医药管理局项目（GZY-GCS-2017-01）：中医药产业发展研究，负责人：刘红宁；国家中医药管理局中医药传承与创新“百千万”人才工程（岐黄工程）项目（国中医药人教函[2018]288号），负责人：刘红宁。