目的 通过构建尿酸性肾病动物模型，检测给药后KIM-1、NGAL、TIMP-1、MCP-1等肾损伤标志物，探讨苗药痛风停汤对尿酸性肾病的保护作用。方法 将70只SD大鼠随机分为空白组、模型组、苗药痛风停高、中、低剂量组、别嘌醇组和苯溴马隆组，构建尿酸性肾病动物模型，造模后各组灌胃给药治疗28天，末次给药后收集大鼠血清及肾脏标本。生化检测大鼠血清尿酸、尿素氮、肌酐含量，用免疫组化法、Western Blot检测肾组织的KIM-1、NGAL、TIMP-1的表达、RT-PCR法检测肾脏组织KIM-1、MCP-1mRNA的表达。结果 生化结果：别嘌醇、高剂量、中剂量、苯溴马隆组组间比较，其BUN、SCR、UA含量差异无统计学差异（P>0.05）。免疫组化结果：①痛风停高、中、低剂量、别嘌醇组KIM-1平均光密度比苯溴马隆组明显升高，差异具有统计学意义（P<0.01）；②痛风停高、中剂量、别嘌醇组NGAL、TIMP-1平均光密度与苯溴马隆组相比，无明显差异（P>0.05）。RT-PCR结果 与别嘌醇及苯溴马隆组相比，痛风停高剂量组KIM-1、MCP-1mRNA表达量与其无明显差异（P>0.05）。Western Blot结果 ①与别嘌醇组相比，痛风停高剂量组KIM-1、NGAL、TIMP-1蛋白表达均与其无明显差异（P>0.05）；②与苯溴马隆组对比，痛风停高剂量组NGAL蛋白表达与其无明显差异（P>0.05）；其余各组KIM-1、NGAL、TIMP-1蛋白表达量升高，差异具有统计学意义（P<0.05）。结论 苗药痛风停汤可显著降低尿酸性肾病大鼠血肌酐、尿素氮及尿酸水平，并可通过降低肾损伤因子KIM-1、NGAL、TIMP-1、MCP-1的表达，从而实现保护肾脏功能作用。

Objective We constructed the animal models of uric acid nephropathy, detected the markers of kidney injury factors such as KIM-1, NGAL, TIMP-1, MCP-1, etc., in order to explore the protective effect of Miao medicine Tongfengting on uric acid nephropathy.Methods 70 SD rats were randomly divided into Tongfengting low dose group,medium dose group and high dose group,blank group, model group,allopurinol group, and benzbromarone group. The animal models of uric acid nephropathy was constructed. Each group was given intragastric administration for 28 days,and the blank group and model group witch were given equal volume of distilled water, The erum and kidney specimens were collected after the last administration. The contents of serum uric acid, urea nitrogen and creatinine were detected by biochemical method. The expression of KIM-1, NGAL and TIMP-1 in kidney tissue was detected by immunohistochemistry and Western Blot, and the expression of KIM-1 and MCP-1mRNA in kidney tissue was detected by RTPCR.Results Biochemical results. Make comparisons between the allopurinol, high-dose, middle-dose, and benzbromarone groups, there was no significant difference in the contents of BUN, SCR and UA (P>0.05). Immunohistochemistry results: ①Compared with the benzbromarone group, The average optical densities of KIM-1 in the high, middle, low-dose and allopurinol groups were significantly increased, and the differences were statistically significant (P<0.01); ②Make comparisons between the allopurinol, high-dose, middle-dose and benzbromarone groups, there was no significant difference in the average optical densities of NGAL and TIMP-1 (P>0.05). RT-PCR results. Make comparisons between the allopurinol, high-dose, and benzbromarone groups, there was no significant difference in the expressions of KIM-1 and MCP-1 Mrna (P>0.05). Western Blot results. ①There was no significant difference in the protein expression of KIM-1, NGAL, TIMP-1 in the high-dose group and the allopurinol group (P>0.05); ②There was no significant difference in the protein expression of NGAL in the high-dose group and he benzbromarone group (P>0.05); Compared with the benzbromarone group, the protein expression of KIM-1, NGAL and TIMP-1 in the other groups were significantly increased, and the differences were statistically significant (P<0.05).Conclusion The Miao medicine Tongfengting can significantly reduce the serum levels of creatinine, urea nitrogen and uric acid in uric acid nephropathy rats, and protect kidney function by reducing the expressions of kidney injury factors KIM-1, NGAL, TIMP-1, and MCP-1.

国家科学技术部国家重点研发计划项目（2017YFC1703904）：民族医药发掘整理与学术传承研究，负责人：张艺；贵州省科学技术厅平台项目（黔科合平台人オ[2020]2202号）：贵州省中医风湿免疫病临床研究中心，负责人：马武开；贵州省科学技术？？基金项目（黔科合基础[2020]1Y361）：基于Wnt信号介导的细胞自噬探讨黑骨藤提取物治疗类风湿关节炎的分子机制，负责人：杨玉涛；贵州省中医药管理局中医药、民族医药科学技术研究课题（QZYY-2021-108）：苗药痛风停方调控ZFP36信号通路治疗痛风的分子机制研究，负责人：曹跃朋。最多挂靠3个基金，请作者确认。