目的 激素是调控植物活性次生代谢产物合成途径的核心调控因子，内源激素结构多样、含量极低，检测难度高，缺乏高效、准确的检测方法。本研究利用超高效液相色谱串联质谱（UHPLC-MS/MS）技术构建了对黄花蒿中茉莉酸、细胞分裂素、赤霉素、生长素、脱落酸等多类植物内源性激素高效定量检测方法。方法 使用Agilent Poroshell 120 EC-C18（2.7 μm， 3.0×150 mm）色谱柱，流动相为0.05%甲酸水-乙腈溶液，梯度洗脱，采用多反应监测模式验证方法可行性，并对黄花蒿不同器官中植物激素进行定量分析。结果 本方法中检测的16种植物激素均呈良好线性，加样回收率、精密度和稳定性均符合植物样品的测定要求。利用此方法测定青蒿不同器官的激素含量，结果表明脱落酸、茉莉酸、茉莉酸甲酯均在叶中含量较高，赤霉素和生长素在根部含量高，细胞分裂素在叶和茎中含量高。结论 青蒿不同器官的激素测定结果可进一步印证脱落酸、茉莉酸、茉莉酸甲酯在调控青蒿素合成中起着重要作用的相关报道，同时表明该方法检测性良好。本文构建的植物内源性激素的检测方法为进一步探究植物生长发育、抵御外界胁迫的相关机制及次生代谢产物的合成等方面提供了手段。

Objective To establish a quantitative detection method of 16 endogenous plant hormones including jasmin, cytokinin, gibberellin, auxin, abscisic acid and their derivatives in Artemisia annua L. by using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method.Methods Agilent Poroshell 120 EC-C18 (2.7 μm, 3.0×150 mm) chromatographic column was chosen. The mobile phase was 0.05% formic acid water-acetonitrile solution. Gradient elution, multi-reaction monitoring mode was used for quantitative analysis of plant hormones in Artemisia annua to verify the feasibility of this method.Results Fine linearity of each hormone of the method was discovered, and the recovery rate, precision and stability were all in line with the determination requirements of plant samples. The method was used to determine the hormone content in different organs of Artemisia annua. The results showed that abscisic acid, jasmonic acid, and methyl jasmonate were all highly accumulated in the leaves, gibberellin and auxin had the highest content in the roots, and the high content of cytokinin was observed in the leaves and followed in stems.Conclusion The results of hormone determination in different organs of Artemisia annua can further confirm the relevant reports that abscisic acid, jasmonic acid, and methyl jasmonate play an important role in regulating the synthesis of artemisinin and the development of leaves and glandular trichomes. At the same time, we represent a sensitive method for hormones detection that might be suitable for a wide range of medicinal plants, which further provides a means to explore the relevant mechanisms of plant growth and development, resistance to stress, and the regulation of secondary metabolites biosynthesis.

国家科学技术部国家重点研发计划（2019YFC1711102）：中药物种功能基因组挖掘，负责人：陈万生。