目的 本研究使用生物信息学方法鉴定黄连WRKY基因家族成员并对其表达模式进行分析，为进一步研究黄连WRKY基因家族的功能及其对黄连生物碱合成途径的调控机制提供参考。方法 利用HMMER、BLAST、TBtools、IQ-Tree等软件，ExPASy、WOLF PSORT、MEME在线网站等对黄连基因组中的WRKY基因家族进行鉴定、可视化分析和表达模式分析。结果 从黄连基因组中鉴定出了41个WRKY基因，其ORF长度为252-2796 bp，编码蛋白的氨基酸数量为84-931 aa，分子质量为9649.32-103620.60 Da，等电点为4.96-10.17，95%以上蛋白预测亚细胞定位于细胞核；41个CcWRKY（黄连WRKY）基因不均一地分布在9条染色体上；系统发育分析将41个CcWRKY蛋白分为3大类，第Ⅰ类有6个，第Ⅱ类有31个，第Ⅲ类有4个；同一类CcWRKY蛋白结构域高度保守，大多CcWRKY基因外显子数量为3-7，内含子数量为2-6。表达分析结果为，除了保守结构域高度缺失的CcWRKY38、CcWRKY40外，所有的CcWRKY都至少在一个组织中有表达，且多数是特异性表达。结论 全面分析了黄连基因组中的WRKY基因家族，有助于进一步解析WRKY基因家族在黄连生物碱生物合成途径的调控机制。

Objective To identify the WRKY gene family members of C. chinensis by bioinformatics methods and analyze its expression patterns , so as to provide reference for further study on the function of WRKY gene family and its regulation pattern on alkaloid synthesis pathway of C. chinensis.Methods Using HMMER, BLAST, TBtools, IQ-Tree and other softwares, ExPASy, WOLF PSORT, MEME online websites, etc, the WRKY gene family in C. chinensis genome was identified, visually analyzed and the expression pattern was analyzed.Results 41 members of the WRKY gene family were identified from the genome of C. chinensis. The length of ORF ranged from 252 bp to 2796 bp, the number of amino acids encoding the protein ranged from 84 aa to 931 aa, the molecular weight ranged from 9649.32 to 103620.60 Da, and the isoelectric point ranged from 4.96 to 10.17. More than 95% of proteins predicted subcellular localization in the nucleus; 41 CcWRKY (C. chinensis WRKY) genes were distributed inhomogeneously on 9 chromosomes. Phylogenetic analysis showed that 41 CcWRKY proteins could be divided into 3 groups, including 6 in class I, 31 in class II and 4 in class III. The same type of CcWRKY protein domain was highly conserved, and the number of exons and introns of most CcWRKY genes was 3-7 and 2-6. Expression analysis showed that all CcWRKY were expressed in at least one tissue, and most of them were specific, except CcWRKY38 and CcWRKY40, which were highly deficient in conserved domains.Conclusion The comprehensive analysis of WRKY gene family in C. chinensis genome is helpful to further elucidate the regulation mechanism of WRKY gene family in C. chinensis alkaloid biosynthesis pathway.

国家自然科学基金项目（面上项目，重点项目，重大项目）