目的 鉴定与丹参酮合成相关转录因子AP2/ERF家族中SmERF108转录因子，并分析转录因子SmERF108的靶基因。方法 利用生物信息学在线分析平台如NCBI、PFAM等分析SmERF108的序列特征；MEGA-X软件用于构建SmERF108与不同功能转录因子的系统进化树；拟南芥原生质体转化法鉴定SmERF108蛋白的亚细胞定位；通过实时荧光定量PCR（Real-time qPCR）对SmERF108基因在丹参不同器官和组织差异表达进行检测；利用酵母体系对SmERF108的转录激活活性进行探究并用酵母单杂技术确定其靶基因。结果 SmERF108具有典型的AP2/ERF保守结构域，属于ERF-B3亚组，系统进化树和保守基序分析显示SmERF108与丹参中SmERF128、青蒿中AaERF2亲缘关系较近且保守基序分布一致；亚细胞定位显示SmERF108蛋白定位于细胞核；SmERF108基因在周皮中表达最高，且呈现周皮（R1）>韧皮部（R2）>木质部（R3）的规律；酵母自激活验证SmERF108具有转录激活活性，同时酵母单杂交确认其能与关键酶基因SmCPS1启动子结合。结论 鉴定到丹参中一个新转录因子SmERF108，生物信息学分析及差异表达分析预测与丹参酮合成相关，分子互作初步证实靶基因为SmCPS1二萜环化酶。

Objective To identify the SmERF108 transcription factor in the AP2/ERF family of Salvia miltiorrhiza related to tanshinone synthesis and to analyze its target gene.Methods Online bioinformatic analysis platforms such as NCBI and PFAM were used to analyze the sequence feature of SmERF108. MEGA-X software was applied to construct phylogenetic trees using protein sequences of SmERF108 and other functional transcription factors. In addition, protoplast transformation in Arabidopsis thaliana was performed to identify the subcellular localization of SmERF108 protein. The differential expression of SmERF108 gene in different organs and tissues of Salvia miltiorrhiza was detected by real-time qPCR. The transcriptional activation activity of SmERF108 was investigated by yeast system and its target gene was identified by yeast monohybrid technique.Results SmERF108 had a typical AP2/ERF conserved domain and belonged to ERF-B3 subgroup. Phylogenetic tree and conserved motifs analyses showed that SmERF108 was closely related to SmERF128 from S. miltiorrhiza and AaERF2 from Arabidopsis thaliana; and their conserved motifs distribution was consistent. Subcellular localization showed that SmERF108 protein was localized in the nucleus. The expression of SmERF108 gene was the highest in the periderm, and the expression of SmERF108 gen was the highest in periderm (R1), followed by phloem (R2), and the lowest in xylem (R3). Yeast self-activation assay showed that SmERF108 had transcriptional activation activity, and yeast one-hybrid confirmed the key enzyme gene SmCPS1 as the target.Conclusion A novel transcription factor SmERF108 is identified from S. miltiorrhiza. Bioinformatics analysis and differential expression analysis show that it may be related to tanshinone synthesis. Molecular interaction preliminarily confirms that the target gene is the diterpene cyclase SmCPS1.

国家自然科学基金委员会青年基金（82003895）：丹参“优形优质”相关转录因子NAC36的分子机制解析，负责人：季爱加；广东省基础与应用基础研究基金委员会广东省基础与应用基础研究基金项目（2019A1515110594）：茉莉酸介导的SmERF128调控二萜合成分子机制研究，负责人：季爱加。