目的 采用SCoT分子标记技术评价青钱柳的遗传多样性。方法 采集广西、湖南、湖北、江西、安徽和贵州6省22个居群共176份样本，对不同居群青钱柳的遗传多样性进行研究。结果 筛选出了5条引物，并对176个样品进行PCR扩增，扩增出总条带数69条，其中多态性条带数69条，多态性百分比（PPB）为100%。有效等位基因（Ne）为1.4912，Nei''s基因多样性指数（H）为0.3017，Shannon''s信息指数（I）为0.4662，种质间基因分化系数（Gst）为0.3742，基因流（Nm）为0.8362。NTSYS聚类分析结果表明，地域和种质是影响青钱柳样品间遗传相似度的重要因素，同一种质、同一地域的样品亲缘关系更为接近。结论 不同的青钱柳居群遗传多样性丰富，其中广西桂林全州县野生和广西柳州三江县栽培的遗传多样性最高，具有很高的进化潜力。地域和种质（野生或栽培）是影响青钱柳各居群间亲缘关系的重要因素，除此之外，还有海拔、土壤、气候、水质、光照、甚至微生物等因素互相叠加，共同对青钱柳产生影响，从而造成青钱柳种质资源的遗传多样性复杂多样。该结果为青钱柳良种选育及质量评价提供科学依据。

Objective To evaluate the genetic diversity of Cyclocarya Paliurus (batal.) Iljinsk using SCoT molecular marker technology.Methods A total of 176 samples from 22 populations in 6 provinces of Guangxi, Hunan, Hubei, Jiangxi, Anhui and Guizhou were collected to study the genetic diversity of Cyclocarya Paliurus in different populations.Results Five primers were screened out, and 176 samples were amplified by PCR. The total number of amplified bands was 69, including 69 polymorphic bands, and the percentage of polymorphism (PPB) was 100%. Effective allele (Ne) was 1.4912, Nei''s gene diversity index (H) was 0.3017, Shannon''s information index (I) was 0.4662, gene differentiation coefficient (Gst) between germplasm was 0.3742, and gene flow (Nm) was 0.8362. The results of NTSYS cluster analysis showed that the region and germplasm were important factors affecting the genetic similarity among the samples of C. chinensis, and the samples with the same germplasm and the same region were more closely related.Conclusion The different populations of Cyclocarya Paliurus are rich in genetic diversity. Among them, Quanzhou County, Guilin, Guangxi, and Sanjiang County, Guangxi, have the highest genetic diversity and have high evolution potential. Region and germplasm (wild or cultivated) are important factors that affect the genetic relationship among the populations of Cyclocarya Paliurus. In addition, factors such as altitude, soil, climate, water quality, light, and even microorganisms are superimposed on each other, which together have an impact on Cyclocarya Paliurus, resulting in the complex and diverse genetic diversity of Cyclocarya Paliurus germplasm resources. The results provide a scientific basis for the selection and quality evaluation of Cyclocarya Paliurus varieties.

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广西中医药大学一流学科建设项目（2018XK031）：广西不同居群青钱柳的DNA条形码研究；中国中医科学院中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目”（2060302）：巴戟天“紫肉”与“优质”形成的分子机制，负责人：田慧。