目的 建立银屑病血热证和血瘀证大鼠模型,评价两个证型间的动物模型差异。方法 血热证银屑病大鼠模型采用复方热药水煎液灌服形成血热证体征,联合咪喹莫特诱导银屑病造模；血瘀证（气虚血瘀证）银屑病则采用疲劳、饥饿、寒湿、惊慌等多因素模拟血瘀证候形成,联合咪喹莫特诱导银屑病样皮炎。观察两证型银屑病空白组及模型组中医证候和银屑病皮损情况,造模结束后取材完成病理组织检测、脾指数计算、血液流变学及关键基因表达水平测定。结果 血热证模型组大鼠在证候表现、PASI评分、Baker评分、脾指数、血浆黏度与空白组相比差异均有统计学意义(P＜0.05)。血瘀证模型组大鼠则在证候表现、体重、PASI评分、Baker评分、脾指数、全血中切、全血低切、血浆黏度方面与空白组存在统计学差异(P＜0.05)。各证型组间关键基因表达水平均具差异。结论 本实验构建的动物模型具备银屑病典型特征且符合中医血热证和血瘀证证候体征,提示两证型银屑病大鼠模型构建成功,这将为阐释中医药辨证论治银屑病的科学内涵提供模型基础。

Objective To establish and compare rat models of psoriasis with blood-heat syndrome and blood-stasis syndrome. Methods The blood-heat syndrome model was induced by compound heat potion decoction and imiquimod. The blood-stasis syndrome(Qi deficiency and blood stasis syndrome) model was induced by fatigue, hunger, cold dampness, panic and imiquimod. The blank group received no intervention. The groups were evaluated for TCM syndromes, psoriasis lesions, pathological tissue, spleen index, hemorheology and key gene expression levels. Result The blood-heat syndrome model group showed significant differences from the blank group in syndrome manifestations, PASI score, Baker score, spleen index and plasma viscosity (P<0.05). The blood-stasis model group showed significant differences from the blank group in syndrome manifestations, body weight, PASI score, Baker score, spleen index, whole blood medium cut, whole blood low cut and plasma viscosity (P<0.05). The key gene expression levels were different among all syndrome groups. The key gene expression levels were different among all syndrome groups. Conclusion The animal models had the typical features of psoriasis and matched the signs of blood-heat syndrome and blood-stasis syndrome of TCM. The models can provide a basis for explaining the scientific meaning of TCM syndrome differentiation in treating psoriasis.

国家自然科学基金青年科学基金