目的 探讨蒙药吉如很古日古木-7（JG-7）和广枣三味汤（GZ-3）预处理对小鼠心肌缺血再灌注损伤（Myocardial ischemia-reperfusion injury，MIRI）的保护作用。方法 将60只雄性C57BL/6J小鼠随机分为假手术（Sham）组、模型（Model）组、复方丹参滴丸（CDDP）阳性对照组、JG-7组、GZ-3组，每组12只建立MIRI模型，H9C2细胞随机分为Control（常氧）组、H/R（缺氧6h复氧14h）组、H/R+JG-7组、H/R+GZ-3组。各组小鼠缺血30min，再灌注24h、7d后进行心功能指标检测，TTC染色检测MIRI 24h后梗死面积，HE染色检测MIRI 24h后观察心肌组织结构和细胞形态，TUNEL细胞凋亡试剂盒检测MIRI 24h 心肌细胞凋亡情况，Masson染色检测MIRI 7d后心肌纤维化情况，腹主动脉取血，分离血清，检测各组小鼠MIRI氧化应激后指标，CCK-8法检测各组H9C2细胞H/R后存活率。结果 TTC结果显示JG-7、GZ-3减少小鼠MIRI 24h后梗死面积。ELISA及试剂盒检测证明JG-7、GZ-3降低MIRI 24h、7d后肌酸磷酸激酶同工酶（CreatineKinase-MB , CK-MB）、乳酸脱氢酶（Lactic dehydrogenase , LDH）、丙二醛（Malondialdehyde , MDA）水平，增加超氧化物歧化酶（Superoxide dismutase , SOD）水平。HE染色结果显示JG-7、GZ-3可改善MIRI 24h后心肌病理改变。TUNEL细胞凋亡检测结果显示JG-7、GZ-3可改善MIRI 24h后心肌组织细胞凋亡情况。Masson染色结果显示JG-7、GZ-3可缩小MIRI 7d后心肌组织纤维化面积。CCK-8法结果显示JG-7、GZ-3可提高H9C2细胞H/R后细胞存活率。结论 蒙药吉如很古日古木-7和广枣三味汤预处理可降低缺血再灌注（ Ischemia-reperfusion，I/R）后造成的损伤，减少小鼠I/R后心肌梗死面积及纤维化情况，保护心脏。

Objective To explore the protective effects of pretreatment with the Mongolian medicine Jiruhen Gurigumu-7 (JG-7) and Guangzao Sanwei Tang (GZ-3) on myocardial ischemia-reperfusion injury (MIRI) in mice. Methods 60 male C57BL/6J mice were randomly divided into sham operation (Sham) group, model (Model) group, compound danshen drip pill (CDDP) positive control group, JG-7 group, GZ-3 group, and 12 mice in each group to establish the MIRI model, and the H9C2 cells were randomly divided into Control (normoxic) group, H/R (hypoxia 6h reoxygenation 14h) group, H/R +JG-7 group, H/R+GZ-3 group. The mice in each group were tested for cardiac function indexes after 30 min of ischemia, 24h and 7d of reperfusion, TTC staining to detect infarct area after 24h of MIRI, HE staining to detect myocardial tissue structure and cellular morphology after 24h of MIRI, TUNEL apoptosis kit to detect apoptosis of myocardial cells after 24h of MIRI, Masson staining to detect myocardial fibrosis after 7d of MIRI, and abdominal aortic sampling to detect fibrosis. fibrosis, blood was taken from the abdominal aorta, serum was separated, and the indexes after oxidative stress of MIRI were detected in each group of mice, and the survival rate of H9C2 cells after H/R was detected in each group by CCK-8 method. Results The results of TTC showed that JG-7 and GZ-3 reduced the infarct area after 24h of MIRI in mice. ELISA and kit assays proved that JG-7 and GZ-3 reduced creatine phosphokinase isoenzyme (CreatineKinase-MB , CK-MB), Lactic dehydrogenase (LDH), malondialdehyde (MDA) levels, and increased superoxide dismutase (SOD) levels. HE staining showed that JG-7 and GZ-3 improved myocardial pathology after MIRI 24 h. TUNEL apoptosis assay showed that JG-7 and GZ-3 improved cardiomyopathic changes after MIRI 24 h and improve myocardial tissue apoptosis after MIRI 24h. Masson staining results showed that JG-7 and GZ-3 could reduce the area of myocardial tissue fibrosis after MIRI 7d. CCK-8 assay results showed that JG-7 and GZ-3 could improve the cell survival rate after H/R in H9C2 cells. Conclusion Pre-treatment with Mongolian medicine Jiruhen Gurigumu-7 and Guangzao Sanwei Tang can reduce the damage caused after ischemia-reperfusion (I/R), decrease the area of myocardial infarction and fibrosis after I/R in mice, and protect the heart.

2022年度草原英才工程青年创新创业—层次项目,内蒙古自治区留学回区人员创新启动支持计划项目