目的? 探讨薏人口服液对肺癌小鼠的抗肿瘤作用。方法? 观察肿瘤组织病理形态；流式细胞仪检测脾脏淋巴细胞CD4+T、CD8+T水平变化；Elisa检测免疫球蛋白IgA、IgG、IL-2、IFN-γ含量；检测血常规；试剂盒测定各组小鼠肝肾谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和丙二醛(MDA)的含量；测定各组小鼠血清中谷丙转氨酶（ALT）、碱性磷酸酶（ALB）、谷草转氨酶（AST）、尿素氮（BUN）、血肌酐（CRE）等指标。转录组找到差异基因并进行通路富集。结果? 各给药组肿瘤组织病理形态改变明显，异型性逐渐降低。与顺铂组相比，薏人组CD4+T、CD8+T、CD4+T/CD8+T、IL-2、IFN-γ、IgG水平显著上升（P＜0.01）；薏人组的IL-2、IgA水平显著上升（p＜0.01）；血常规结果，与模型组相比，顺铂组WBC、RBC、HGB、PLT、Lym%和GR%水平显著下降（P＜0.01）；与顺铂组相比，薏人组WBC、RBC、PLT、HGB、Lym%水平显著上升（P＜0.01）。肝肾的抗氧化指标表明，薏人组的肝脏、肾脏组织中GSH、SOD水平显著上升（P＜0.01），MDA水平显著下降（P＜0.01）。对小鼠肝肾功指标的影响，薏人组AST、ALT、BUN、CRE显著下降（P＜0.01），薏人组ALB水平显著上升（P＜0.01）。转录组结果，薏人高剂量组主要通过影响TNF信号通路和IL-17信号通路发挥抗肿瘤作用。结论? 高剂量薏人口服液主要通过抑制IL-17和TNF信号通路，抑制肿瘤增殖，减轻炎症反应，增强肺癌小鼠免疫力，改善血常规和减轻肝肾损伤。

Objective:To investigate the anti-tumor effect of Job"s tears oral solution on lung cancer mice. Methods: Observe the histopathological morphology of the tumor; flow cytometry detected the changes in the levels of CD4+T and CD8+T in splenic lymphocytes; Elisa detected the contents of immunoglobulins IgA, IgG, IL-2 and IFN-γ; blood routine was detected; the kit determined the levels of liver and kidney glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and malondialdehyde ( MDA) content; serum alanine aminotransferase (ALT), alkaline phosphatase (ALB), azelaic transaminase (AST), urea nitrogen (BUN), and blood creatinine (CRE) were measured in each group of mice. The transcriptome was found differential genes and pathway enrichment was performed. RESULTS: Tumor histopathological and morphological changes were obvious in each administration group, and the heterogeneity was gradually reduced. Compared with the cisplatin group, the levels of CD4+T, CD8+T, CD4+T/CD8+T, IL-2, IFN-γ, and IgG in the CAU group were significantly increased (p < 0.01); the levels of IL-2 and IgA in the CAU group were significantly increased (p < 0.01); blood routine results: compared with the model group, the WBC, RBC, HGB, PLT, Lym%, and GR% in the cisplatin group The blood routine results: compared with the model group, WBC, RBC, HGB, PLT, Lym% and GR% in the CAU group decreased significantly (p<0.01); compared with the cisplatin group, WBC, RBC, PLT, HGB and Lym% in the CAU group increased significantly (p<0.01). The antioxidant indexes of liver and kidney showed that the levels of GSH and SOD in the liver and kidney tissues of Job"s tears group increased significantly (P＜0.01), and the level of MDA decreased significantly (P＜0.01). Effects on liver and kidney function indexes in mice: AST, ALT, BUN and CRE decreased significantly in the CAU group (P＜0.01), and ALB level increased significantly in the CAU group (P＜0.01). Transcriptome results, CAU high-dose group mainly exerted anti-tumor effects by affecting TNF signaling pathway and IL-17 signaling pathway. CONCLUSION: High-dose Job"s tears oral solution inhibits tumor proliferation, attenuates inflammatory response, enhances immunity, improves blood routine and reduces liver and kidney injury in lung cancer mice mainly by inhibiting IL-17 and TNF signaling pathway.

哈尔滨市制造业科技创新人才项目(2022CXRCCG014)；2024黑龙江省“双一流”学科协同创新成果项目(LJGXCG2024-P09)